Ebola-HIV-RNA viruses

Ebola-HIV-RNA viruses.

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Ebola-HIV-RNA viruses

This could also be relevant for Ebola, which also is a single-stranded RNA virus:

HIV vaccine

The body’s initial “vaccine-reaction” against HIV could be prolonged if the viruses were inactivated. In comparison, the poliovirus can be inactivated by cleavage of its RNA by ammonia. Hopefully, the same can be true for HIV. Both viruses contain single-stranded RNA and proteases. Maybe the proteases perform (catalyse) aminolysis and cut the RNA strands. HIV has even RNase H which is specialized in breaking RNA strands. The phosphates (PO4-) in RNA repel water more than ammonia, and they are thus favoring aminolysis over hydrolysis. (NH3 is a stronger nucleophile than H2O).

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC354068/?page=1

The tight encapsulation/packing of RNA in the viruses will probably promote the aminolysis process. The hydrophobic cores in the ribonucleoproteins will attract ammonia.

And the host cells use RNases to hydrolyse foreign RNA. Maybe the RNases switch to aminolysis when ammonia is available, and thereby speed up this defense.

In this regard, inhalation of ammonia could be a way to destroy HIV and other RNA viruses in blood and tissue.

http://chemwiki.ucdavis.edu/Organic_Chemistry/Organic_Chemistry_With_a_Biological_Emphasis/Chapter_10%3A_Phosphoryl_transfer_reactions/Section_10.4%3A_Phosphate_diesters

Written by:

Ottar Stensvold

Molde 
Norway

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Red Bull

Caffeine + Glucose.bmp

The Red Bull effect
Caffeine and glucose (also called grape sugar) has each a well-known effect.
Caffeine acts particularly in the brain by blocking the receptor(s) for the inhibitory neurotransmitter adenosine.
The adenosine level increases by physical efforts and by the time we are awake.
The caffeine molecule resembles the adenosine molecule.
Maybe the effect of caffeine and glucose can be enhanced by creation of a stuff that resembles adenosine even more
than caffeine alone does, in a reaction between caffeine and glucose.
It should make up a stuff with an effect stronger than obtainable by the usual table sugar (the disaccharide sucrose) + coffee/caffeine.
The Red Bull drink contains both caffeine and glucose, and the reason why this drink works so well could be a chemical reaction between glucose and caffeine.
Chemically it is fully feasible that there will be created a compound which resembles adenosine more than caffeine alone does.
Thus there will be a stronger effect, ie a better blockage of the receptor(s).
See picture attachment – it shows the common formation of a glycosidic bond,
where nitrogen attaches to the anomeric carbon in a nucleophilic substitution reaction. Acidic pH will catalyze this reaction.

I have not tried to blend coffee and glucose. I don`t know the health risks, not even for the legal Red Bull drink.
Please be responsible.
 
Sincerely,

Ottar Stensvold

Molde
Norway

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Carbon capture

The carbonic anhydrase enzymes as means of carbon fixation?

Carbonic anhydrase is an enzyme that assists rapid inter-conversion of carbon dioxide and water into carbonic acid, protons and bicarbonate ions.

CO2 + H2O <=> HCO3-  +  H+  (in high pH and high CO2 concentration mostly to the right)

They are widespread in nature, being found in animals, plants, algae and certain bacteria.

I tested this enzyme by churning some flowers from the garden in water, and added carbonated drink.

The bubbling stopped immediately, and I managed to precipitate some matter when adding salt. (That`s how corals, CaCO3, Na2CO3x10H2O, etc. are formed!)

Since these enzymes are extremely efficient, soluble and abundant, there is a fair chance they can be harvested and used in carbon capture. They can even be enhanced by genetic modification.

Ideally, the exhaust from a car can be fluxed through a water bath containing this enzyme and some Ca++ or Na+ in optimized pH. And the hundred-and-thirty-something grams of CO2 per km can be dumped as solid rock!

Sincerely,

Ottar Stensvold

Molde

Norway

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Burning of ammonia – environmental

Can ammonia save the Earth? 
Huge amounts of ammonia are continuously released into the atmosphere. 
To harvest this and burn it instead of fossil hydrocarbons, can be a way to reduce greenhouse gas emissions. 
It might be the perfect combination of hydrogen and nitrogen economy: 

4NH3 + 3O2 = 2N2 + 6H2O (g)  

energy released = –1267.20 kJ/mol  

(Wikipedia)

To aminate hydrocarbons, e.g. diesel oil, is relatively simple,and can be achieved by means of air efflux from decaying organic matter. One can also use consentrated ammonia; I did so and got a solution which burned intensely.

-CH2- + NH3 + 1/2 O2 = -CH-NH2- + H2O (or H2)

It should be possible to accumulate a “fishbone” of amines in long-chain hydrocarbons. This “explosive” could resemble nitroglyserine in dynamite or the toxic but clean-burning rocket fuel hydrazine (NH2-NH2). Or for worse: the dangerously unstable diesel bomb (diesel + ammonium nitrate, NH4NO3) used by terrorists.  (Smashing of carbon nuclei in a fission reaction giving helium as waste product is a possibility)
During combustion, the diesel component will ignite the ammonia, and ensure an altered, hotter, more pressured, more complete combustion which is more efficient in regard to CO2 and NOx emissions.  When N2 is made, much energy is yielded, due to the strong triple bond formed, and N2 is harmless to the atmosphere. This stuff, this idea, remains to be tested, and eventually engines have to be adjusted. It is urgent, because to stop the  polluting engines seems impossible.
– Sincerely,
Ottar Stensvold
Molde
Norway 
 
 
 

 

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tRNA on Ribosome

tRNA on Ribosome and how to wrap in and stop antigens with nucleic acids
    Around traffic light - Click image to download.          Helsenotis        
New drug  – a chance
 
I found these articles supporting my theory about amino acids` binding to their cognate codons (triplets):
Idea in biochemistry part 1-4:


PART 1

Quick web searches on the topic ribosome all show the same:
the amino acid-loaded tRNA arrives at the ribosome with its
anti-codon first. I think this could be an oversimplification, which
doesn`t take notice to the probable, intuitive fact that the gene is
a blueprint for the protein, a mirror-copy made through evolution.
Say the proteins initially instructed the genes and not vice versa.
Say the food came first, and then the means to carry it.

For such a complex process as translation to proceed without many errors,
there has to be another, more primary mechanism of proofreading. Maybe there is a
closer link between amino acids and the mRNA than previously thought.

Maybe there is a transient key-lock binding between the amino acid and
the codon before the anticodon binds.
The bases, adenine (A), guanine (G), uracil (U), thymine (T), and cytosine (C) seems to reach for the amino acids, and the first and the second
bases seem to play the greatest role. For instance, the short one-cyclic U are
in the second (midst) position in codons binding hydrophobic amino acids,
indicating the hydrophobic aa`s R-groups (side chains) will turn downwards/inwards (towards the codons, away from the water) when binding.
And hydrophilic amino acids have the longer two-cyclic A in the midst position
in their respective codons, indicating the R-groups will turn upwards/outwards, away from the codons,
towards the water when encountering the codons.

My point is that it should be possible to make any short gene sequence
that could match any protein, almost as an antibody. The oligonucleotides
will be shrunk at point of binding, and they will probably work for only shorter 
stretches. As far as I know it should not be too dangerous to apply in vivo,
but of course animal tests, research etc. are needed. 
I read for instance in Discover Magazine and at Nature.com that eosinophilic cells 
catapult mitochondrial DNA nets against parasites (malaria).

It should be possible to target any protein-markers in any disease,
and hence destroy the culprits. Say, put a virus in a PCR machine and make
cheap copies of tailored antibodies! Or use the new oligo synthesis factories!

I try to explain my theory in this video:
http://www.youtube.com/watch?v=7834CXASQF8 

I found these articles supporting my theory about amino acids` binding to their cognate codons:

http://www.ncbi.nlm.nih.gov/pubmed/14561881?dopt=Abstract’,’NCBI’,’700′,’400′)

Sincerely,

Ottar Stensvold,

Reinhold Zieglers veg 14 B

6414 Molde
Norway
mobile: +47 95 177 433
epost: ottarstensvold@hotmail.com

web: http://ottarstensvold.spaces.live.com

born:  February 7th 1971
——————————————

PART 2

A drop of ordinary water-smear on a microscopy slide reveals a pattern:
the bacteria in the water lines up, symbolized like this:  o…..o..o…o…o
These lines are best seen at 400x magnification. See example picture at bottom of this page.
My theory / idea is that DNA from ruptured bacteria binds and absorbs proteins
from its own species.
So what? If so, if a nucleic acid can bind to a protein in a coded manner,
then the aptamer technology is the tech of the future.
Say for instance the codon GUG, which codes for the amino acid valine, weakly
binds this amino acid. Symbolized like this: E-<
The codon-amino acid binding gives several combinatorial possibilities with
regard to polar interactions, mechanic fit and R-group placement.
If we can make aptamers tailored to bind specific proteins, then we can
take down any pathogen.
—-
PART 3
 
The amino acid sequence of the HIV surface protein GP 120
can be found at GenBank:

http://www.ncbi.nlm.nih.gov/protein/7769644?ordinalpos=1&itool=EntrezSystem2.PEntrez.Sequence.Sequence_ResultsPanel.Sequence_RVDocSum

My plan is to try to make an array of different aptamers to hit this protein.
The approach I will use is basically to design aptamers that are based on
the amino acids` corresponding codons. In the DNA-aptamers, I will replace
U with T, and in the third wobble-position I will choose the assumed least
interfering base (e.g. T, i.e. Thymine). RNA aptamers can also be used.
There is a chance that the aptamers will match directly.
The matches have to be at accessible parts of the protein`s surface.
The aptamers will be ordered (outsourced), for instance from:

http://www.biomers.net/upload/Image/Preislisten/DNA_En_EUR.pdf


or from Eurogentec in San Diego, California:

http://www.eurogentec.com/news/106-eurogentec-launches-new-oligo-synthesis-facility.html

 
The price of the aptamers will be maybe around one tenth of comparable drugs,
i.e. monoclonal antibodies.

The stock of different aptamers will be annotated at my portable computer.

Then, step 2, we have to beg institutions which store HIV viruses to test the aptamers in vitro.
The results can be viewed with electron microscopes.

(One can also test aptamers on oncoproteins in stored tissue samples,
e.g. the 17 aa-sequence in the CD44 protein which is over-expressed on membranes on glioma cancer brain cells.)

This project needs help from media. The headlines could be:

“Burn off money to test HIV drug”
“Find needle in haystack”

Press: “Will this be tested in humans? Will there be marketing? Sales?
Investing?”

Answer:  “We will find a balanced solution, it`s fair that developed
countries pay for some of the venture.”

The task will require all communication skills and business intelligence.

It is a bit like cloud computing: once the hidden codes are found,
they have to be administered.

It is my goal to reach this goal of my life!

—————————————— 

 
PART 4
 
The aptamer:

GGTTGGTGTGGTTGG
(or ggttggtgtggttgg)

 
or GGT TGG  TGT   GGT  TGG
or GGU UGG UGU GGU UGG  (in RNA) corresponds to the amino acids:
gly     trp    cys     gly   trp   abbreviations:
G      W        C      G      W

This aptamer bind to the blood coagulation factor protein thrombin.
I found it at:
http://bloodjournal.hematologylibrary.org/cgi/content/abstract/83/3/677?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&fulltext=aptamer&searchid=1&FIRSTINDEX=0&sortspec=relevance&resourcetype=HWCIT

To find the amino acid sequence in thrombin,  I search GenBank at:
http://www.ncbi.nlm.nih.gov/nuccore/169808403?ordinalpos=1&itool=EntrezSystem2.PEntrez.Sequence.Sequence_ResultsPanel.Sequence_RVDocSum

The combination “GW” occurred once in the thrombin protein,
the combination “WG” occurred twice,
the combination “WC” occurred twice,
the combination “GC” occurred twice,
and the combination “CG” occurred three times.

The latter combination, for instance, should then bind to the aptamer-codons UGU GGU.

I did not find similar matches in the comparable plasma proteins albumin or insulin,
and I think further research will find no other similar matches either.
It is striking that the aptamer comprises the range of codons encoding the protein (aa range) it binds!
 
The numbers of possibilities to get a twosome aa-combination of a pool of twenty different amino acids,
should plainly speaking be 1/20*1/20 = 1/400,
and this makes specific binding likely!
 
This underscores my suggestion that proteins can bind specific to their codon combinations in mRNA or sense (+)ssDNA.
In the June 2009 Scientific American issue, there is an article about silent mutations (by Chamary and Hurst)
which highlights that different codons encoding the same amino acids give alterations in the proteins.
It`s thus feasible that this bias is caused by the codons` positioning of the amino acids (R-group placement in space). Such a moulding will give a hand-glove fit which can be harnessed in aptamer-drug design.

For pictures of bacteria in water, see this site: http://iceberg.dri.edu/blogs4.php

 

Sincerely,

Ottar Stensvold

Molde

Norway

(Regarding autoimmune MS and diabetes:
My idea is to take down the protein markers
which the T-cells home in on when they perform autoimmunity, and/or target the receptors /
surface proteins on the T-cells which they use in this task. At GenBank we can find some of
the needed gene/protein sequences.)

I try to explain my theory in this video:
http://www.youtube.com/watch?v=7834CXASQF8

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Booster Sports Drops (in English)

 
Booster Sports Drops
 
I present here the nutrient I have developed, for general info in the first place.

I think there is a great opportunity in applying energy to the immune system. The stuff described here under (polyacetylated carbohydrates / sugar derivatives found in plants) will as all nutrients first encounter the immune cells when taken up to blood. It will even directly fuel the immune cells by diffusion into the mucous membranes in the lymphatic tissue in and around the tonsils.

It thus implies great opportunities not only by means of nutritional energy supply, but also by means of worldwide distribution and sales.

“and the leaves of the tree were for the healing of the nations” – Revelation 22:2.

——————- 

Booster Sports Drops Rum is made of sugar, vinegar and rum aroma. Colour may vary. Store cold and dry. Seize up pieces. 

——————-

Ordering from abroad of Norway: 
Booster Sports Drops Rum will be sent directly to your address in a 90 gram letter-package for NOK 250,- freight and works included. 
Please transfer NOK 250 to the bank account: xxxxxxxxxxx SWIFT code SPARNO22 (six capitals and two digits) 
(Sparebanken Møre, Storgata 33, 6413 Molde, Norway) 
 
Write clearly your name and full delivery address as payment information. 
Delivery time depends on where in the world you are. Please be patient. 
(in case of delivery failure, the money will be remitted) 
The Drops Factory boostersportsdrops.com 
Reinhold Zieglers veg 14 B, 6414 Molde, Norway 
phone: +47 95 177 433 

bank account: xxxxxxxxxxx SWIFT code SPARNO22 (Sparebanken Møre, Storgata 33, 6413 Molde, Norway) 

org.nr: xxx xxx xxx, email:

owner: Ottar Stensvold 
Factory opens as soon as locality is found! 
 
 
 
the booster sports drops
natural nutraceutical boooooster
 
Ladies and gentlemen, let me introduce the world-class nutraceutical: the booster sports drops. It`s an invention based on the precious experience made by the sugar plantation slaves. During their suffering in the heat of the day, they drank / ate raw cane sugar and experienced an enduring stream of energy. This made them capable to bear inhuman burdens of work. The secret lay in the acetylated sugar compounds in the canes. I will honour the memory of these good people and avoid slavery and unfair treatment happen again. Therefore, I invented the booster sports drops. I discovered this kind of sugar compound that can access the cells in the body without the help of insulin. Insulin is a hormone that in a complex receptor-ligand-cascade chain lets the cells express glucose transporters as GLUT-4 (and other transporter / receptor proteins like for instance LDL-receptors) in their membranes, e.g. muscle cells. This sugar compound is less polar and more branched than ordinary glucose, and thus more easily bind to the unpolar cell membrane (softens it). At the interface of the cell membranes, it also solutes glucose.The cells will simply engulf this by endocytosis. Other nutrients, as for example cholesterol (in LDL) will also be taken up in the process. This scavenges the blood, lowering the glucose rate and the cholesterol rate in the blood, – key factors for a healthy life! It thus functions as a generic insulin / glucose transporter, and might help diabetics among others. The sugar compound in the booster sports drops promotes endocytosis. Once taken up by the cells, the complex carbohydrates (in endosomes) will be attached by lysosomes in the cytoplasm. The acidic (low pH) lysosome vesicles contain acid hydrolases. These enzymes hydrolyse the sugar compounds and release new functional molecules, namely acetic acids which otherwise can`t enter cells in such packages. In a normal human cell, there are around 2000 mitochondria (more in muscle cells). The mitochondria are the energy factories of the body. Acetic acid enter the mitochondria and goes directly into a high yield, O2-consuming energy production. (Search web for pictures of citric acid cycle.) This process ignites the whole body, and keeps the metabolism, the burning of nutrients, -“the wheel of life” in motion. Since the sugar in the booster sports drops are evenly distributed and don`t discriminate between cells, it restores a physiological balance. Acetylation increases bioavailability and crossing of the blood-brain barrier. Luckely, it let the most healthy cells utilize, thrive and work. One can for instance assume that cancer cells, which have an insuffient respiratory chain, and mainly survive by the incomplete, anaerobic glycolysis (breakdown of glucose) which occurs in the cytoplasm, get suppressed when other healthy nearby cells thrive by respiration (i.e. immune cells). And maybe the cancer cells lack the efficient lysosomal machinery to avoid engorgement / molecule overload / sensitivity for metabolism.The same can be true for other diseases as well (bacterial cells). And consider treatment of diseases which occur because of too strong immune reactions; overactive autoimmune phagocytes can be kept busy by the drug (complex sugar derivatives) instead of destroying surrounding tissue for food / debris (e.g. arthritis and allergy / asthma). Triggering of naive regulatory T cells is another possibility. There may be other possible benefits as well. For instance could metabolic changes lead to changes in skin which in turn can lead to less acne. It makes the endothel of the circulatory system more resilient, and can decrease blood pressure by app. 5%. And maybe deployment in membranes of erythrocytes and neurons will increase flexibility and give a more rapid exchange of CO2 / O2 and neurotransmitters. Actually, the nervous system use one third of the body`s energy and should benefit from any good sugar-supply! The booster sports drops is the ideal drops for any high-performer, whether it`s the brain worker, the athlete or the creative artist. It lights the fire! The way to synthesise this sugar compounds and how to make the drops are of course secret. It`s even not completely understood what`s happening in the process, and the exact molecular formulas are only predicted, but they certainly work! The way these nutraceutical carbohydrate compounds act in the body have always been naturally occurring. There are also drugs, like auranofin (Ridaura) or aspirin that contains ester-bonded acetic acid, and which mechanism of action has not been fully revealed yet. Along with the slaves, it`s also appropriate to mention Jesus who got the strengthening vinegar in his last fight, suffering at the cross (John 19). Now is this healing energy source revealed, elucidated and fortified for the first time! The booster sports drops does not contain alcohol, but it has a wonderful blended taste of Jamaica rum and caramel. And the flavour? -It`s like dew beside a river in the Norwegian spring forest.
Please search web for explanatory videos and pictures of cell membranes, endocytosis, auranofin molecule, aspirin molecule, glucose molecule etc.

– see this 35-second video about endocytosis:
http://www.youtube.com/watch?v=4gLtk8Yc1Zc 

A three-cyclic sugar / carbohydrate compound, with acetic acid attached, are probably present in the booster sports drops. (picture shown under)

Ac=Acetate=CH3CO2- – get dynamically released in the cytoplasm by the help of acid hydrolases.

Polyphenols, like for instance resveratrol (found in red wine) or curcumin (found in turmeric), or quercetin – a flavonoid in fruits / apples and other polyphenols (e.g in green tea), can maybe act upon cell membranes in a similar fashion as the poly-cyclic molecule in the booster sports drops. Please search web for info about resveratrol, curcumin, quercetin and polyphenols / flavonoids / antioxidants. See also cinnamaldehyde / cinnamon in this regard.

Picture of polyacetylated complex carbohydrate molecule probably present in the Booster Sports Drops:

 
 
 
 
 
Picture showing principle of glucose absorption into cells:
http://www.powersupplements.com/ala/0254824.html
The sugar-derivative in the booster sports drops can interact with / substitute / supplement some of the stuff / transporters shown at right in this picture. -Note that there are also other isoforms of glucosetransporters which are not dependent upon insulin for their expression, e.g. in brain cells (not shown here).
Regarding sugar cane plantation slaves, see picture:
http://www.movinghere.org.uk/galleries/roots/caribbean/images/S0001575.jpg
Mitochondrion -see picture:

The end product adenosine triphosphate (ATP) in mitochondria is an energy carrier molecule which play a key role in cell communication and activity. The oxidative phosporylation to form ATP occurs in the electron transport chain where oxygen (1/2 O2) are reduced to form water, H2O, by the joining of two protons (2H+) and two electrons (2e-). Please search web for info about citric acid cycle! The pivotal citric acid cycle takes place in mitochondria.The quick, initiating, igniting, efficient fuel in this cycle is acetate or acetyl (CH3CO): Acetate + Coenzyme A (CoA-SH) + ATP => Acetyl-CoA + ADP. The stuff (free acetate) bypasses both glycolysis and the extremely complicated pyruvate dehydrogenase complex by the help of the enzyme Acetyl-CoA Synthetase to become Acetyl-CoA! 
Please search the internet for more info about formation and hydrolysis of esters.
  
 
Written by / invented by Ottar Stensvold
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