Ebola-HIV-RNA viruses
This could also be relevant for Ebola, which also is a single-stranded RNA virus:
HIV vaccine
The body’s initial “vaccine-reaction” against HIV could be prolonged if the viruses were inactivated. In comparison, the poliovirus can be inactivated by cleavage of its RNA by ammonia. Hopefully, the same can be true for HIV. Both viruses contain single-stranded RNA and proteases. Maybe the proteases perform (catalyse) aminolysis and cut the RNA strands. HIV has even RNase H which is specialized in breaking RNA strands. The phosphates (PO4-) in RNA repel water more than ammonia, and they are thus favoring aminolysis over hydrolysis. (NH3 is a stronger nucleophile than H2O).
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC354068/?page=1
The tight encapsulation/packing of RNA in the viruses will probably promote the aminolysis process. The hydrophobic cores in the ribonucleoproteins will attract ammonia.
And the host cells use RNases to hydrolyse foreign RNA. Maybe the RNases switch to aminolysis when ammonia is available, and thereby speed up this defense.
In this regard, inhalation of ammonia could be a way to destroy HIV and other RNA viruses in blood and tissue.
http://chemwiki.ucdavis.edu/Organic_Chemistry/Organic_Chemistry_With_a_Biological_Emphasis/Chapter_10%3A_Phosphoryl_transfer_reactions/Section_10.4%3A_Phosphate_diesters
Written by:
Ottar Stensvold
Molde
Norway
Red Bull
The Red Bull effect
Caffeine and glucose (also called grape sugar) has each a well-known effect.
Caffeine acts particularly in the brain by blocking the receptor(s) for the inhibitory neurotransmitter adenosine.
The adenosine level increases by physical efforts and by the time we are awake.
The caffeine molecule resembles the adenosine molecule.
Maybe the effect of caffeine and glucose can be enhanced by creation of a stuff that resembles adenosine even more
than caffeine alone does, in a reaction between caffeine and glucose.
It should make up a stuff with an effect stronger than obtainable by the usual table sugar (the disaccharide sucrose) + coffee/caffeine.
The Red Bull drink contains both caffeine and glucose, and the reason why this drink works so well could be a chemical reaction between glucose and caffeine.
Chemically it is fully feasible that there will be created a compound which resembles adenosine more than caffeine alone does.
Thus there will be a stronger effect, ie a better blockage of the receptor(s).
See picture attachment – it shows the common formation of a glycosidic bond,
where nitrogen attaches to the anomeric carbon in a nucleophilic substitution reaction. Acidic pH will catalyze this reaction.
I have not tried to blend coffee and glucose. I don`t know the health risks, not even for the legal Red Bull drink.
Please be responsible.
Sincerely,
Ottar Stensvold
Molde
Norway
Carbon capture
The carbonic anhydrase enzymes as means of carbon fixation?
Carbonic anhydrase is an enzyme that assists rapid inter-conversion of carbon dioxide and water into carbonic acid, protons and bicarbonate ions.
CO2 + H2O <=> HCO3- + H+ (in high pH and high CO2 concentration mostly to the right)
They are widespread in nature, being found in animals, plants, algae and certain bacteria.
I tested this enzyme by churning some flowers from the garden in water, and added carbonated drink.
The bubbling stopped immediately, and I managed to precipitate some matter when adding salt. (That`s how corals, CaCO3, Na2CO3x10H2O, etc. are formed!)
Since these enzymes are extremely efficient, soluble and abundant, there is a fair chance they can be harvested and used in carbon capture. They can even be enhanced by genetic modification.
Ideally, the exhaust from a car can be fluxed through a water bath containing this enzyme and some Ca++ or Na+ in optimized pH. And the hundred-and-thirty-something grams of CO2 per km can be dumped as solid rock!
Sincerely,
Ottar Stensvold
Molde
Norway
Burning of ammonia – environmental
4NH3 + 3O2 = 2N2 + 6H2O (g)
energy released = –1267.20 kJ/mol
(Wikipedia)
To aminate hydrocarbons, e.g. diesel oil, is relatively simple,and can be achieved by means of air efflux from decaying organic matter. One can also use consentrated ammonia; I did so and got a solution which burned intensely.
-CH2- + NH3 + 1/2 O2 = -CH-NH2- + H2O (or H2)
tRNA on Ribosome
PART 1
Quick web searches on the topic ribosome all show the same:
the amino acid-loaded tRNA arrives at the ribosome with its
anti-codon first. I think this could be an oversimplification, which
doesn`t take notice to the probable, intuitive fact that the gene is
a blueprint for the protein, a mirror-copy made through evolution.
Say the proteins initially instructed the genes and not vice versa.
Say the food came first, and then the means to carry it.
For such a complex process as translation to proceed without many errors,
there has to be another, more primary mechanism of proofreading. Maybe there is a
closer link between amino acids and the mRNA than previously thought.
My point is that it should be possible to make any short gene sequence
that could match any protein, almost as an antibody. The oligonucleotides
It should be possible to target any protein-markers in any disease,
and hence destroy the culprits. Say, put a virus in a PCR machine and make
cheap copies of tailored antibodies! Or use the new oligo synthesis factories!
I try to explain my theory in this video:
I found these articles supporting my theory about amino acids` binding to their cognate codons:
http://www.ncbi.nlm.nih.gov/pubmed/14561881?dopt=Abstract’,’NCBI’,’700′,’400′)
Ottar Stensvold,
Reinhold Zieglers veg 14 B
6414 Molde
Norway
mobile: +47 95 177 433
epost: ottarstensvold@hotmail.com
web: http://ottarstensvold.spaces.live.com
born: February 7th 1971
——————————————
A drop of ordinary water-smear on a microscopy slide reveals a pattern:
the bacteria in the water lines up, symbolized like this: o…..o..o…o…o
These lines are best seen at 400x magnification. See example picture at bottom of this page.
My theory / idea is that DNA from ruptured bacteria binds and absorbs proteins
from its own species.
So what? If so, if a nucleic acid can bind to a protein in a coded manner,
then the aptamer technology is the tech of the future.
Say for instance the codon GUG, which codes for the amino acid valine, weakly
binds this amino acid. Symbolized like this: E-<
The codon-amino acid binding gives several combinatorial possibilities with
regard to polar interactions, mechanic fit and R-group placement.
If we can make aptamers tailored to bind specific proteins, then we can
take down any pathogen.
PART 3
can be found at GenBank: http://www.ncbi.nlm.nih.gov/protein/7769644?ordinalpos=1&itool=EntrezSystem2.PEntrez.Sequence.Sequence_ResultsPanel.Sequence_RVDocSum
My plan is to try to make an array of different aptamers to hit this protein.
The approach I will use is basically to design aptamers that are based on
the amino acids` corresponding codons. In the DNA-aptamers, I will replace
U with T, and in the third wobble-position I will choose the assumed least
interfering base (e.g. T, i.e. Thymine). RNA aptamers can also be used.
There is a chance that the aptamers will match directly.
The matches have to be at accessible parts of the protein`s surface.
The aptamers will be ordered (outsourced), for instance from: http://www.biomers.net/upload/Image/Preislisten/DNA_En_EUR.pdf
or from Eurogentec in San Diego, California:
http://www.eurogentec.com/news/106-eurogentec-launches-new-oligo-synthesis-facility.html
i.e. monoclonal antibodies.
The stock of different aptamers will be annotated at my portable computer.
Then, step 2, we have to beg institutions which store HIV viruses to test the aptamers in vitro.
The results can be viewed with electron microscopes.
e.g. the 17 aa-sequence in the CD44 protein which is over-expressed on membranes on glioma cancer brain cells.)
This project needs help from media. The headlines could be:
“Burn off money to test HIV drug”
“Find needle in haystack”
Press: “Will this be tested in humans? Will there be marketing? Sales?
Investing?”
Answer: “We will find a balanced solution, it`s fair that developed
countries pay for some of the venture.”
The task will require all communication skills and business intelligence.
It is a bit like cloud computing: once the hidden codes are found,
they have to be administered.
It is my goal to reach this goal of my life!
——————————————
GGTTGGTGTGGTTGG
(or ggttggtgtggttgg)
or GGU UGG UGU GGU UGG (in RNA) corresponds to the amino acids:
gly trp cys gly trp abbreviations:
G W C G W
This aptamer bind to the blood coagulation factor protein thrombin.
I found it at:
To find the amino acid sequence in thrombin, I search GenBank at:
http://www.ncbi.nlm.nih.gov/nuccore/169808403?ordinalpos=1&itool=EntrezSystem2.PEntrez.Sequence.Sequence_ResultsPanel.Sequence_RVDocSum
The combination “GW” occurred once in the thrombin protein,
the combination “WG” occurred twice,
the combination “WC” occurred twice,
the combination “GC” occurred twice,
and the combination “CG” occurred three times.
I did not find similar matches in the comparable plasma proteins albumin or insulin,
and I think further research will find no other similar matches either.
For pictures of bacteria in water, see this site: http://iceberg.dri.edu/blogs4.php
Sincerely,
Ottar Stensvold
Molde
Norway
(Regarding autoimmune MS and diabetes:
My idea is to take down the protein markers
which the T-cells home in on when they perform autoimmunity, and/or target the receptors /
surface proteins on the T-cells which they use in this task. At GenBank we can find some of
the needed gene/protein sequences.)
I try to explain my theory in this video:
http://www.youtube.com/watch?v=7834CXASQF8
Booster Sports Drops (in English)
I think there is a great opportunity in applying energy to the immune system. The stuff described here under (polyacetylated carbohydrates / sugar derivatives found in plants) will as all nutrients first encounter the immune cells when taken up to blood. It will even directly fuel the immune cells by diffusion into the mucous membranes in the lymphatic tissue in and around the tonsils.
It thus implies great opportunities not only by means of nutritional energy supply, but also by means of worldwide distribution and sales.
“and the leaves of the tree were for the healing of the nations” – Revelation 22:2.
——————-
Booster Sports Drops Rum is made of sugar, vinegar and rum aroma. Colour may vary. Store cold and dry. Seize up pieces.
——————-
bank account: xxxxxxxxxxx SWIFT code SPARNO22 (Sparebanken Møre, Storgata 33, 6413 Molde, Norway)
org.nr: xxx xxx xxx, email:
– see this 35-second video about endocytosis:
http://www.youtube.com/watch?v=4gLtk8Yc1Zc
Ac=Acetate=CH3CO2- – get dynamically released in the cytoplasm by the help of acid hydrolases.
Polyphenols, like for instance resveratrol (found in red wine) or curcumin (found in turmeric), or quercetin – a flavonoid in fruits / apples and other polyphenols (e.g in green tea), can maybe act upon cell membranes in a similar fashion as the poly-cyclic molecule in the booster sports drops. Please search web for info about resveratrol, curcumin, quercetin and polyphenols / flavonoids / antioxidants. See also cinnamaldehyde / cinnamon in this regard.
Picture of polyacetylated complex carbohydrate molecule probably present in the Booster Sports Drops:
http://www.powersupplements.com/ala/0254824.html
The sugar-derivative in the booster sports drops can interact with / substitute / supplement some of the stuff / transporters shown at right in this picture. -Note that there are also other isoforms of glucosetransporters which are not dependent upon insulin for their expression, e.g. in brain cells (not shown here).
http://www.movinghere.org.uk/galleries/roots/caribbean/images/S0001575.jpg
The end product adenosine triphosphate (ATP) in mitochondria is an energy carrier molecule which play a key role in cell communication and activity. The oxidative phosporylation to form ATP occurs in the electron transport chain where oxygen (1/2 O2) are reduced to form water, H2O, by the joining of two protons (2H+) and two electrons (2e-). Please search web for info about citric acid cycle! The pivotal citric acid cycle takes place in mitochondria.The quick, initiating, igniting, efficient fuel in this cycle is acetate or acetyl (CH3CO): Acetate + Coenzyme A (CoA-SH) + ATP => Acetyl-CoA + ADP. The stuff (free acetate) bypasses both glycolysis and the extremely complicated pyruvate dehydrogenase complex by the help of the enzyme Acetyl-CoA Synthetase to become Acetyl-CoA!
Booster Sports Drops (norsk)
Verdensnyhet fra Molde!
Booster Sports Drops
Fabrikken åpner så snart lokale er skaffet!
Det er et nytt helseprodukt som er laget etter modell fra råsukker. I saften fra sukkerrør finnes en sukker-molekylfamilie som er vanlig i planter. Det er mer komplekse karbonforbindelser enn vanlig sukker (glukose), med mange korte karbonfragmenter festet til. Disse gir blant annet rom-aroma, HCO2CH2CH3. (Rom lages av råsukker). Men viktigst i metabolsk sammenheng er acetyl, CH3CO2-, eller eddiksyre som er et sentralt mellomprodukt i cellenes sukker -og fettnedbrytning. I tusener av år har alt fra pirater til sukkerplantasjeslaver nyttiggjort seg dette spesielle sukkerstoff. Nå er det for første gang oppkonsentrert i en ny synteseprosess hvor eddiksyre festes til komplekse, klebrige karbon bærermolekyler ved esterifisering. For å gjøre en lang historie kort og lettfattelig, kan dropsenes virkemåte illustreres slik:
Dette sukkerstoff bindes lett til cellemembraner og opptas hurtig. Inne i cellene blir eddiksyre frigjort og trekker inn i mitokondriene, som er våre energigeneratorer. I mitokondriene blir eddiksyren forbrent. Dette gir den ultimate helsegevinst; allerede i munn og svelg diffunderer stoffet gjennom slimhinnene og styrker celler: epitelceller, lymfocytter, mastceller, makrofager etc. Videre gir dropsene energi til celler i mage/tarm-veggen, celler som forbruker svært mye energi. Og etter opptak i blod vil alle kroppens celler dra nytte av denne sukkerforbindelsen, – alt fra immunceller til muskelceller og hjerneceller. Foruten prestasjons-fremmende effekter, er helseeffekten vel så viktig, bl.a. mht bekjempelse av infeksjoner. I denne “glemte”, men mer naturlige biokjemiske reaksjonsvei allokeres energien mer effektivt til kroppens celler. Og best av alt: det er et rent, naturlig næringsmiddel med en fantastisk god smak og aroma!
Booster Sports Drops Rum fremstilles av sukker, eddik og aroma. Dropsene tilsettes ikke kunstig fargestoff, så det vil være en naturlig fargevariasjon.
Lagres tørt og kaldt.
Slik bestiller De:
Bruk bankoverføring via Deres nettbank, velg “giro med melding” (ikke KID)
Velg beløpet De vil bestille for (se priser under)
NB! Skriv Deres navn og leveringsadresse i meldingsfeltet for bankoverføringen.
Overfør til bankkontonummer xxxxxxxxxxx (Sparebanken Møre, Molde)
De vil motta produktet direkte i Deres postkasse, vanligvis etter 3-4 virkedager.
Sender over hele Norge
(Brevgiro kan også brukes, men da må lengre leveringstid påregnes)
Priser:
50 Kr = 35 gram Booster
100 Kr = 70 gram Booster
150 Kr = 105 gram Booster
200 Kr = 140 gram Booster
osv.
(ved eventuelle leveringsvanskeligheter vil beløpet bli refundert)
Dropsfabrikken boostersportsdrops.com
organisasjonsnummer: xxx xxx xxx,
epost:
innehaver:
Ottar Stensvold
Booster Sports Drops Rum – like viktig som det daglige brød!
booster@boostersportsdrops.com
Dropsfabrikken Booster Sports Drops
Malaria
MALARIA:
Does natron (or similar compounds) work against malaria mosquitoes?
– a proposal for health and economic growth.
I read at Wikipedia that natron (Na2CO3) was an ancient household insecticide. I don`t know how it works, but it can possibly somehow act on the carbonic anhydrase (CA) enzymes in red blood cells (and from plant feed), and thus interfere in the CO2 / CO3–/ H+ / O2 cycle. It has a wide geological occurrence, is cheap, environmentally friendly and worth a try! Mosquitoes live on blood and nectar, and natron can maybe impede their digestion. Maybe the gut bursts as CA transforms HCO3- and H+ to CO2 and H2O, before the digestive enzymes break CA down!
And since the mosquitoes lay their eggs in lakes, and natron exists in arid water beds, it should be natural to spread it on water surfaces. Female mosquitoes hunt their blood host by detecting carbon dioxide, and an increase in this stuff should lure them.
After further reading, I found that mosquitoes and their larvae produce their own carbonic anhydrase enzymes in the gut. Their anterior gut has a high pH, and the enzymes` main function is to convert CO2 to H2CO3. By adding Na2CO3, the enzymes will work in reverse, producing much CO2 which hopefully could destroy the mosquitoes.
Sincerely,
Ottar Stensvold
Molde
Norway
e-mail:
ottarstensvold@hotmail.com
mobile: +47 95 177 433
Virology – treating HIV and other viral infections
N-CR-C-N-CR-C-N-CR-C-N-CR-C … and so on.
These protein chains are densely packed.
C-C-C-CO2-.
This acid is small enough to creep in between the protein chains, it will intermingle and
get locked between the R-groups in the protein chains.
The acid will act as a soap molecule, make fractures and fringe, puncture / implode the viruses.
Beside acting as an amphipathic soap molecule in the fat / protein-intersection, the butyric acid and its related molecules could neutralize the viruses.
Maybe around 10% are under 12 carbon atoms` length.
When butter is boiled in base (HO-) the fatty acids get released (saponification).
When I boiled butter in sodium bicarbonate (NaHCO3 – baking soda – natron) and water, I got a cocktail of water-soluble, short-chain, free fatty acids. The cocktail showed very promising features in dissolving stacked proteins.
When drinking this in sufficient amounts, some of the free fatty acids will avoid getting built into chylomicrons in the enterocytes. Thus they will be interspersed in blood and lymph, and possibly they will help dissolve the viruses.
One spoon of butter, two spoons of baking soda and three spoons of water boiled to the top in a small pot will do the trick.
Ottar Stensvold
Reinhold Zieglers veg 14 B
6414 Molde
Norway
e-mail: ottarstensvold@hotmail.com
Ottarstensvold's Blog 
